Dietary cotton‐seed oil attenuates secretion of adhesion molecules by Human Umbilical Vein Endothelial Cells (HUVEC)

Abstract

We investigated the effect of post‐prandial HDL isolated from healthy females consuming a single high‐fat meal rich in cotton seed oil (CSO) on TNF‐α stimulated soluble Intercellular Adhesion Molecule (sICAM‐1) and Vascular Cell Adhesion Molecule (VCAM‐1) expressions in HUVEC. In a randomized cross‐over design, eleven healthy females consumed 1 of 2 iso‐caloric meals containing either CSO or blended palm oil (BPO) (1 g of fat/kg BW). Blood was drawn at 0, 3 and 6 hrs after meal and HDL isolated by sequential centrifugation. HUVEC incubated with TNF‐α were treated with HDL (2, 4, 8 μg/mL) and protein expression of adhesion molecules secreted were determined by Western blotting. Serum TNF‐α, oxidized LDL, sICAM‐1, and sVCAM‐1 were determined by ELISA. One‐way repeated ANOVA was performed to determine differences between three time periods and two groups. HDL isolated from CSO but not BPO attenuated expression of sICAM‐1 and VCAM‐1, in a dose‐dependent manner. Levels of oxidized LDL in the CSO were significantly lower than the BPO (p<0.05). The CSO‐mediated attenuation of adhesion molecule expression should reduce the adherence of inflammatory cells to the vascular endothelium, thus reducing the risk for cardiovascular disease.Supported by Texas Department of Agriculture