Abstract
Mammalian herbivores have developed numerous adaptations to utilize their plant-based diets including a modified gastrointestinal tract (GIT) and symbiosis with a GIT microbiota that plays a major role in digestion and the maintenance of host health. The red panda (Ailurus fulgens) is a herbivorous carnivore that lacks the specialized GIT common to other herbivores but still relies on microorganisms for survival on its almost entirely bamboo diet. The GIT microbiota is of further importance in young red pandas, as high cub mortality is problematic and has been attributed to failure to meet nutritional requirements. To gain insight into the establishment of the GIT microbiota of red pandas, we examined microbial communities in two individuals following dietary changes associated with weaning using next-generation 16S rRNA Illumina MiSeq paired-end sequencing of faecal samples. Across all four stages (pre-weaning, during weaning, post-weaning and adult), the GIT microbial community displayed low diversity and was dominated by bacteria in the phylum Firmicutes with lesser contributions from the Proteobacteria. A core community was found consistently across all weaning stages and included species within the taxa Escherichia-Shigella, Streptococcus, Clostridium and an unclassified Clostridiaceae. Analysis of the overall community composition and structure showed that although the GIT microbiota is established early in red pandas, dietary changes during weaning further shape the community and are correlated with the presence of new bacterial species. This work is the first analysis of the GIT microbiota for red panda cubs during weaning and provides a framework for understanding how diet and host microbiota impact the development of these threatened animals.
Highlights
Microorganisms play a fundamental role in the survival of their animal hosts (McFall-Ngai et al, 2013)
The gastrointestinal tract (GIT) microbiota is of further importance in young red pandas, as high cub mortality is problematic and has been attributed to failure to meet nutritional requirements
To gain insight into the establishment of the GIT microbiota of red pandas, we examined microbial communities in two individuals following dietary changes associated with weaning using next-generation 16S rRNA Illumina MiSeq paired-end sequencing of faecal samples
Summary
Microorganisms play a fundamental role in the survival of their animal hosts (McFall-Ngai et al, 2013). An unusual exception is the red panda (Ailurus fulgens), which is able to survive on entirely plantderived materials without such adaptations. Red pandas are members of Carnivora but consume an exclusively herbivorous diet (Loeffler, 2011). Unlike typical herbivores, they have the GIT of a carnivore (Davis, 1964) with a simple stomach, no caecum (Davis, 1964; Roberts and Gittleman, 1984), and a shorter digesta transit time (the rate of ingested foods passing through the GIT) on the order of 10 h or less (Bleijenberg and Nijboer, 1989; Nijboer and Dierenfeld, 2011) compared to 48–75 h in traditional hindgut herbivores, such as equines (Cuddeford et al, 1995), and 2–6 h for carnivores, such as canines (Boillat et al, 2010)
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