Dietary Calcium Attenuates Platelet Aggregation and Intracellular Ca 2 Mobilization in Spontaneously Hypertensive Rats

Abstract

Spontaneously hypertensive rats (SHR) are known to be blood pressure sensitive to dietary calcium. The effects of dietary calcium on platelet aggregation and intracellular Ca 2+ mobilization were assessed by turbidimetric methods and fura-2 methods, respectively, in washed platelets of SHR. Ca 2+ ATPase activity was examined in aortic membrane fractions. Six weeks of dietary calcium supplementation attenuated the increase of systolic blood pressure (SBP 199 ± 16 v 170 ± 9 mm Hg, P < .001) and thrombin-induced platelet aggregation (84.5 ± 3.7 v 73.7 ± 7.4%, P < .004) at 9 weeks of age. The ionomycin-induced intracellular calcium ([Ca 2+] i) peak in the absence of external Ca 2+, which reflects [Ca 2+] i storage size, and thrombin-evoked [Ca 2+] i release from [Ca 2+] i storage were decreased by 2.0% Ca diet (472 ± 55 v 370 ± 23 nmol/L, P < .001, 339 ± 29 v 278 ± 33 nmol/L, P < .002). In addition, SBP was positively correlated with platelet aggregation (r = 0.703, P = .0088), thrombin-evoked [Ca 2+] i (r = 0.739, P = .0044), and ionomycin-induced [Ca 2+] i (r = 0.591, P = .0415), respectively. However, there was no significant effect of dietary calcium on Ca 2+-ATPase activity in aortic membranes. These results suggest that dietary calcium supplementation had a beneficial effect on platelets of SHR by attenuating [Ca 2+] i mobilization from [Ca 2+] i storage. The hypotensive effect of dietary calcium might be associated with attenuated [Ca 2+] i mobilization in SHR.