Dietary administration of tyramine upregulates on immune resistance, carbohydrate metabolism, and biogenic amines in Macrobrachium rosenbergii

Abstract

Survival rates of prawn, Macrobrachium rosenbergii, against Lactococcus garvieae, immune parameters, carbohydrate metabolism and biogenic amines were determined when the prawn were fed diets containing tyramine (TA) at the levels of 1 and 10 mg kg−1. Results showed that prawn fed diets containing TA for 3 days, challenged with L. garvieae, and then continuously fed individually tested diets had a significantly higher survival rate than those fed the control diet at 168 h after the challenge, in a dose-dependent manner. Results included significant increases in total haemocyte count (THC), granular cells (GCs), semigranular cells (SGCs), phenoloxidase (PO) activity, respiratory bursts (RBs), RBs per haemocyte, phagocytic activity, and clearance efficiency to L. garvieae. Significantly lower plasma glucose and lactate were observed in prawn fed with TA-containing diets for 3 days, a result consistent with increases in the survival rate of the challenge test and the haemolymph octopamine (OA) level. Haemolymph dopamine (DA), norepinephrine (NE), TA, and OA levels of prawn that were fed TA-supplemented diets increased significantly after 1 day, and OA level increased continuously until the third day with a dose-effect relationship. It is therefore concluded that TA can be absorbed from a TA-containing diet to elevate haemolymph TA level, inducing the release of DA, NE, and OA to maintain homeostasis. The higher, more extensive OA expression promoted carbohydrate metabolism and immune resistance in M. rosenbergii.