Abstract
2´-fucosyllactose (2´-FL) is an abundant human milk oligosaccharide (HMO) in human milk with diverse biological effects. We recently reported ingested 2´-FL stimulates central nervous system (CNS) function, such as hippocampal long term potentiation (LTP) and learning and memory in rats. Conceivably the effect of 2´-FL on CNS function may be via the gut-brain axis (GBA), specifically the vagus nerve, and L-fucose (Fuc) may play a role. This study had two aims: (1) determine if the effect of ingested 2´-FL on the modulation of CNS function is dependent on the integrity of the molecule; and (2) confirm if oral 2´-FL modified hippocampal LTP and associative learning related skills in rats submitted to bilateral subdiaphragmatic vagotomy. Results showed that 2´-FL but not Fuc enhanced LTP, and vagotomy inhibited the effects of oral 2´-FL on LTP and associative learning related paradigms. Taken together, the data show that dietary 2´-FL but not its Fuc moiety affects cognitive domains and improves learning and memory in rats. This effect is dependent on vagus nerve integrity, suggesting GBA plays a role in 2´-FL-mediated cognitive benefits.
Highlights
MethodsSprague Dawley male adult rats (2.5–4 months old; 250–300 g) were supplied by Charles River Laboratories
Data Availability Statement: All relevant data are within the paper
The evolution of field excitatory post-synaptic potential (fEPSP) evoked at the CA3-CA1 synapse by single pulses presented before and after an HFS session was followed in three groups of animals (n = 10 animals per group; Fig 1B): Control, Fuc and 2’-FL
Summary
Sprague Dawley male adult rats (2.5–4 months old; 250–300 g) were supplied by Charles River Laboratories. Rats were housed in pairs and kept on a 12 h light/dark cycle with controlled ambient temperature and humidity of 21.5 ± 1 ̊C and 55 ± 8%, respectively. Food and water were available ad libitum. Animals devoted to vagotomy as well as to LTP studies were kept individually after surgical procedures. Animals (n = 10 per group) were divided in three groups: Control, Fucose, and 2’-FL.
Sign-in/Register to view highlights & summary Sign-in/Register to access full text options 
Free) 
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a call
