Diet supplementation of astaxanthin mitigates cadmium induced negative effects on oxidative, inflammatory and non-specific immune responses, and the intestinal morphology in Trachinotus ovatus

Abstract

Knowledge about additive on alleviating the negative effects of fish exposed to cadmium (Cd) needs to be more identified in the aquaculture. Therefore, the present study aims to investigate whether diet supplemented with astaxanthin could mitigate adverse effects on hepatic Cd deposition, oxidative, inflammatory and non-specific immune responses, and intestinal morphology of Trachinotus ovatus exposed to dietary Cd treatment. Three isonitrogen-isolipid experimental diets supplemented with/without CdCl2/Astaxanthin were formulated to feed the T. ovatus for 30 days. Results demonstrated that considerably higher hepatic Cd level was found in two Cd supplementing groups compared to the control group (p < 0.05), while no statistical difference of hepatic Cd concentration between these two Cd supplementing groups (p > 0.05). The activity of hepatic total superoxide dismutase, RNA expression levels of hepatic Nrf2-keap1 pathway genes, pro-inflammatory genes, and non-specific immune genes were no statistical differences between the control group and the dietary Cd supplementing group (p > 0.05), while these data in the dietary Cd with astaxanthin group showed significantly higher than that in the dietary Cd without astaxanthin group (p < 0.05). On the contrary, hepatic malondialdehyde content in the dietary Cd group showed significantly higher than that in the control group and dietary Cd with astaxanthin group (p < 0.05). Significantly lower mid-intestine morphology parameters were obtained in the dietary Cd group than the control group (p < 0.05), while significantly higher data were found in the dietary Cd with astaxanthin group compared to the dietary Cd group (p < 0.05). These results indicated that astaxanthin could mitigate the inhibitory effects of Cd on the oxidative, inflammatory and non-specific immune responses, and intestinal morphology of T. ovatus while not reduce the hepatic Cd deposition.