Diesel degradation by emulsifying bacteria isolated from soils polluted with weathered petroleum hydrocarbons

Abstract

This study evaluated bacterial populations (total, lipolytic, P-solubilizers, N-fixing free, and hydrocarbonoclastic bacteria) from uncontaminated soils or soils contaminated with weathered petroleum hydrocarbons (WPH) and evaluated the emulsifying and diesel-degrading properties for each bacterial strain. Soils collected from Tabasco State (Mexico) included moderately contaminated (50,000mgkg−1), highly contaminated (150,000mgkg−1), or non-contaminated samples. Bacterial populations showed significant differences (P<0.05) among the three soils, with highly contaminated soil containing the highest populations of lipolytic and phosphate (P) solubilizing bacteria. Thirteen bacterial strains were isolated from non-contaminated and highly contaminated soils. These bacteria were able to degrade diesel and to produce surfactants, as determined by four methods [emulsification index (E24%), cell surface hydrophobicity, drop collapse, and hydrocarbon displacement]. Bacteria with high emulsification activity were molecularly identified (16S rRNA) as Serratia marcescens C11S1, S. marcescens C7S3A, Citrobacter freundii CCC4DS3, Raoultella ornithinolytica C5S3, Stenotrophomonas maltophilia CCC10S1, and St. pavanii C5S3FN. The strain C7S3A showed the highest diesel emulsification (74.2%) and high cell hydrophobicity (173%) and diesel degradation (96%). A consortium containing the six bacterial strains also showed high diesel degradation (97%). The biochemical tests demonstrated that the bacterial strains were also able to produce indole acetic acid. Our results suggest that the emulsifying bacteria may useful for bioremediating soils chronically contaminated with weathered petroleum hydrocarbons.