Abstract
Pseudomonas aeruginosa methylparathion degrading bacterial strain previously isolated and identified in our lab was subjected to confirm their ability to diesel oil degradation in synthetic wastewater containing diesel oil (10 % v/v). They are cultured in modified mineral salts agar medium with diesel oil as the sole source of carbon. They were able to grow and distinguish in 7 days of incubation. Biosurfactant was produced in the medium and its effect was detected using emulsification activity, growth estimation and gravimetric analysis as an indicator for this process. Factors affecting hydrocarbon utilization by this isolate were pH, temperature, salt concentration and agitation along with glucose, peptone, phosphate and nitrate concentrations. Gravimetric and FTIR analysis showed that the strain was able to degrade complex hydrocarbon chains to simple ones. The optimum conditions of various factors showed that, neutral pH, with temperature of 320C, and agitation rate of 150 rpm gave the optimum conditions to accelerate diesel oil degradations, while additional nutrients of glucose (1 mg/l), peptone (5 mg/l), sodium nitrate (2 mg/l), phosphate (7 mg/l) and sodium chloride (3 mg/l) enhanced optimum effect on the bioprocess. The bacterial strain of indigenous pure monoculture having the degradative potential of xenobiotic compounds of various pollutants includes diesel oil as well as methylparathion pesticide practically. The main conclusion is that Pseudomonas aeruginosa dou-1 strain can be able to degrade complex hydrocarbons and make it an ideal applicant in bioremediation.
Highlights
Environmental pollution with petroleum and petroleum products has been recognized as one of the most serious current problems, hydrocarbons may reach the water table before becoming immobilized in the soil
The bacterial strain used for the biotreatment process was Pseudomonas aeruginosa dou-1 (Table.1 and Figure 1), which is a methylparathion degrading bacterial strain previously identified according to Bergy’s manumal of determinative bacteriology (Holt et al, 1994; Cappuccino and Sherman, 2010) stored in the lab was subjected to diesel oil degradation in synthetic wastewater containing diesel oil at 10% (MMSM)
The bacterial strain which was found to persist and grow under high diesel oil concentration was screened from this study and named as Pseudomonas aeruginosa dou-1
Summary
Environmental pollution with petroleum and petroleum products (complex mixture of hydrocarbons) has been recognized as one of the most serious current problems, hydrocarbons may reach the water table before becoming immobilized in the soil. The numbers of spills that have dumped millions of gallons of crude oil into the environment have been steadily increasing over the past decade. Biological methods of rehabilitation of polluted sites represent an interesting alternative These techniques are based on the microorganism’s capacities to degrade petroleum compounds (Harayama, 1999). Pseudomonas aeruginosa mehtylparathion degrading bacterial strain previously identified and stored in our lab was subjected to diesel oil degradation in synthetic wastewater containing diesel oil
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