Dielectrophoretic Manipulation and Separation of Microparticles Using Microarray Dot Electrodes

Bashar Yafouz,Fatimah Ibrahim,Nahrizul Kadri

doi:10.3390/s140406356

Bashar Yafouz, Fatimah Ibrahim + Show 1 more

Open Access

https://doi.org/10.3390/s140406356

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Journal: Sensors (Basel, Switzerland)	Publication Date: Apr 3, 2014
Citations: 104	License type: CC BY 3.0

Affiliation: University of Malaya

Abstract

This paper introduces a dielectrophoretic system for the manipulation and separation of microparticles. The system is composed of five layers and utilizes microarray dot electrodes. We validated our system by conducting size-dependent manipulation and separation experiments on 1, 5 and 15 μm polystyrene particles. Our findings confirm the capability of the proposed device to rapidly and efficiently manipulate and separate microparticles of various dimensions, utilizing positive and negative dielectrophoresis (DEP) effects. Larger size particles were repelled and concentrated in the center of the dot by negative DEP, while the smaller sizes were attracted and collected by the edge of the dot by positive DEP.

Highlights

Particle manipulation and separation techniques have been of interest to many research groups worldwide for various biomedical applications, including cell concentration, separation, patterning, Sensors 2014, 14 trapping and positioning [1]
DEP has been used in research on cell lysis [12] and to characterize a wide range of yeast cells [13] and mammalian cells, such as neurons [14], leukemia cells [15], platelets [16], cancer cells [17], and sperm cells [18]
The simple and rapid fabrication processes of planar electrodes make microarray dot electrode a potential solution to be utilized in research involving the development of cost-effective POC devices

Summary

Introduction

Particle manipulation and separation techniques have been of interest to many research groups worldwide for various biomedical applications, including cell concentration, separation, patterning, Sensors 2014, 14 trapping and positioning [1]. These methods have facilitated the development of cost-effective point-of-care (POC) devices that can rapidly collect, prepare and analyze human biological samples. DEP is a non-invasive method that describes the movement of polarizable particles when subjected to a non-uniform electric field. DEP has been used in research on cell lysis [12] and to characterize a wide range of yeast cells [13] and mammalian cells, such as neurons [14], leukemia cells [15], platelets [16], cancer cells [17], and sperm cells [18]

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Results

Conclusion