Die Reaktion von Kalli and Mesophyllprotoplasten von Vicia faba L. and Vicia narbonensis L. auf H2O2

Abstract

Summary Comparative investigations on Vicia faba and Vicia narbonensis demonstrate that young callus cultures derived from leaves, roots and shoots, wounded leaves and protoplasts isolated from precultured leaves of Vicia faba show a strong reaction to H2O2 (red colour). This effect is correlated to the presence of L-dopa in these tissues and cells. Calluses and mesophyllprotoplasts from Vicia narbonensis do not contain L-dopa and do not react to H2O2 with the formation of a red coloured substance. No, respectively only a very slight reaction to H2O2 is visible in healthy leaves and protoplasts isolated from fresh (without preculture) leaves of Vicia faba, though L-dopa is detectable. Obviously additionally an activation of polyphenoloxidases (by wounding, induction of callus growth) is necessary to form red dopachrome. In this system the presence of activated enzyme might be immediately made visible in situ so far L-dopa is present.