Dideoxy sequencing and structural analysis of the rat insulin-like growth factor binding protein-1 gene

Abstract

Insulin-like growth factor binding protein-1 (IGFBP-1) is an important modulator of IGF bioavailability. To facilitate studies of IGFBP-1 regulation and function in rodent models, we cloned the rat IGFBP-1 gene and analyzed its structure by dideoxy sequencing. The rat IGFBP-1 gene is relatively small (∼ 5 kb) and contains 4 exons and 3 introns, similar to the human IGFBP-1 gene.