Abstract
Identification of motor and sensory nerves is important in applications such as nerve injury repair. Conventional practice relies on time consuming staining methods for this purpose. Here, we use laser scanning infrared differential interference contrast (IR-DIC) microscopy for label-free observation of the two types of nerve. Ventral and dorsal nerve roots of adult beagle dogs were collected and sections of different thicknesses were imaged with an IR-DIC microscope. Different texture patterns of the IR-DIC images of the motor and sensory nerve can be distinguished when the section thickness increases to 40[Formula: see text][Formula: see text]m. This suggests that nerve fibers in motor and sensory nerves have different distribution patterns. The result hints a potential new way for more rapid identification of nerve type in peripheral nerve repair surgery.
Highlights
Peripheral nerve system contains motor and sensory nerves, which are classied according to the type of the majority nervebers inside
At 40 m thick, the internal nervebers in the ventral nerve root present as stripe shapes, while those in the dorsal nerve root still look like circular shapes (Figs. 2(a) and 2(b))
Most nervebers look like short stripes, some show as circular shapes (Figs. 1(a) and 1(b))
Summary
Peripheral nerve system contains motor and sensory nerves, which are classied according to the type of the majority nervebers inside. For trauma patients with severe peripheral nerve injuries, nerve repair surgery may be required to reconnect divided nerve. To get the best outcome of the repair surgery, it is essential to discriminate motor and sensory nerve bundles and make correct connections between the distal and proximal part of the same type of injured nerve bundle.[1,2]. Identication using electrical stimulation has been applied. With microneurography technique, ̄ne needles are inserted into peripheral nerves and patients are stimulated at sensory and motor nervebers and reported sensations.[5] This method, requires the patients to remain conscious as the injuries are fresh. Staining based on di®erent chemical compositions, such as acetylcholinesterase (AChE) histochemical staining, are applied.[6,7,8,9,10] the procedure is time consuming due to the trace amount of the targeting constituents and the slow staining process
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