DIBENZOCYCLOOCTADIENE LIGNANS ISOLATED FROM THE ROOTS OF SCHISANDRA CAULIFLORA AND THEIR NITRIC OXIDE INHIBITION ACTIVITY ON LPS STIMULATED RAW 264.7 CELLS

Abstract

Objectives: To isolate and determine the chemical structure of some compounds from the roots of Schisandra Cauliflora and their inhibitory activity on nitric oxide production. Methods: The roots of Schisandra cauliflora, which were collected in Vinh Phuc in April 2022, were processed before being extracted and isolated to obtain compounds. Compounds structures were elucidated based on extensive spectroscopic analyses, including 1D- and 2D-nuclear magnetic resonance spectroscopy (NMR), mass spectrometry (HR-ESI-MS), and CD spectra. The obtained compounds were evaluated for inhibitory activity on nitric oxide production on LPS stimulated RAW 264.7 cells. Results and conclusion: From the methanol extract of the roots of Schisandra cauliflora, three dibenzocyclooctadiene lignans were isolated, including ananonin J, ananolignan F, and ananolignan C. Their structures were elucidated. All the compounds inhibited nitric oxide productions on LPS stimulated RAW 264.7 cells with IC50 values of 45.24 ± 1.46, 41.32 ± 1.45, and 48.71 ± 1.34µM, respectively, compared to that of the positive control of dexamethasone (IC50 14.20 ± 0.54µM). This is the first time these lignan compounds have been isolated from the roots of Schisandra cauliflora.