Diazotroph and arbuscular mycorrhizal fungal diversity and community composition responses to karst and non-karst soils

Abstract

Diazotrophs and arbuscular mycorrhizal fungi (AMF) are important functional microbes that provide and transport nutrients to plants. Diazotroph and AMF diversity and community composition were investigated in karst mature forests (MFs) and non-karst forests, as well as in croplands (CRs) as a reference. Diazotroph diversity and community composition significantly shifted in MFs between karst and non-karst soils, with a higher diazotroph richness and Shannon index in karst MFs than in non-karst soils. Similar levels of AMF diversity and community composition were observed between karst and non-karst CRs and MFs. According to the co-occurrence network analysis results, the pairs of correlation links within the diazotroph and AMF groups were higher in karst MFs than in non-karst MFs, whereas this interaction was lower in karst CRs than in non-karst CRs. The diazotroph genera Bradyrhizobium and Anabaena and the AMF genera Glomus and Rhizophagus were mostly correlated with other species in both karst and non-karst soils. Diazotrophs and AMF communities were mainly affected available nutrients (e.g., nitrogen and phosphorus) and environmental factors (e.g., temperature and precipitation), respectively. Soil calcium content was an important factor that affected the distributions of diazotrophs and AMF between karst and non-karst soils. Overall, our study suggested that the increased interactions between diazotrophs and AMF in karst forests, as compared with those in non-karst soil, led to the stability of the diazotrophs and AMF co-occurrence patterns for maintaining nutrients that are effectively absorbed and utilized. Thus, strengthening the cooperative relationships between diazotrophs and AMF are important and necessary in calcareous soils during natural vegetation restoration.