Diazepam- and chlordiazepoxide-mediated increases in erythrocyte aldehyde dehydrogenase activity and its possible implications

Abstract

Erythrocyte ALDH activity was assayed in alcoholic ( n = 70) and nonalcoholic ( n = 40) subjects. In general, alcoholics without any prior medications ( n = 57) were found to have a decreased ALDH activity (mean ± SD: 3.38 ± 1.7 mU; p < 0.001) as compared to control group (5.10 ± 1.57 mU). However, a group of alcoholics who were detoxified with benzodiazepines ( n = 13) prior to blood collection for enzyme assay were found to have higher ALDH activity (4.92 ± 2.46 mU; p < 0.05) as compared to alcoholics who were not detoxified. In vitro experiments demonstrated that both diazepam (DZM) and chlordiazepoxide (CDP) could activate the ALDH. The magnitude of enzyme activation by DZM and CDP appear to correlate with their relative potency of tranquilizing effect. Further, the observed ability of DZM to reverse the inhibition of ALDH mediated by disulfiram may explain the biochemical basis of the reported ability of benzodiazepines (BDZ) to reduce the intensity of disulfiram ethanol reaction (DER).