Abstract
DIATOM material freshly gathered during February 1946 from Chichester Harbour was identified as Navicula ramosissima (Agardh) Cleve. The organism was arranged in files within a filamentous muco-gelatinous sheath forming frondose colonies up to 10 cm. in length. When the filaments were placed in diluted sea-water, differences in osmotic pressure caused the diatom cells to be extruded from the envelope. These cells were taken up with a sterilized pipette and allowed to fall upon the following nutrient agar medium Medium 1): disodium phosphate 0.02 gm., sodium nitrate 0.10 gm., soil extract 50 c.c., agar 10 gm., sea-water 1,000 c.c. The colonies were at first very slow to develop, but after several days cell division proceeded fairly regularly for about a week, after which the colonies became quiescent and showed signs of failing.
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