Abstract
Laccase is a versatile multicopper oxidase that holds great promise for many biotechnological applications. For such applications, it is essential to explore good biocatalytic systems for high activity and recyclability. The feasibility of membrane enclosed enzymatic catalysis (MEEC) for enzyme recycling with laccase was evaluated. The dialysis membrane enclosed laccase catalysis (DMELC) was tested for the conversion of the non-phenolic model substrate 2,2′-Azino-bis(3-ethylbenzthiazoline-6-sulfonate) (ABTS). Trametes versicolor laccase was found to be completely retained by the dialysis membrane during the process. The ABTS total conversion after DMELC reached the same values as the batch reaction of the enzyme in solution. The efficiency of DMELC conversion of ABTS under different process conditions including shaking speed, temperature, ABTS concentration and pH was investigated. The repetitive dialysis minimally affected the activity and the protein content of the enclosed laccase. DMELC retained 70.3 ± 0.8% of its initial conversion after 5 cycles. The usefulness of MEEC extends to other enzymes with the benefit of superior activity of an enzyme in solution and the recyclability which is normally only obtained with immobilized enzymes.
Highlights
Enzymatic bioconversion can be accomplished under mild conditions, achieving high reaction specificity and rates, and generally only requires a small amount of biocatalyst (Nguyen et al 2014)
No laccase activity and protein concentration was detected in the solution outside the dialysis membrane over a period of 48 h
dialysis membrane enclosed laccase catalysis (DMELC) is a feasible biocatalysis approach The results demonstrated that laccase was completely retained in the dialysis membrane due to the high molecular weight of laccase and that no significant leakage occurred within the timeframe of the experiments
Summary
Enzymatic bioconversion can be accomplished under mild conditions, achieving high reaction specificity and rates, and generally only requires a small amount of biocatalyst (Nguyen et al 2014). Enzymatic processes have been developed in the food industry, fine chemicals synthesis, wastewater treatment and bioremediation (Rios et al 2004). Batch reactions with enzymes in solution are often utilized and, most of the time, require relatively simple conditions to be controlled in the process. Enzyme recycling increases the catalytic productivity of the enzymes by reusing them for several batches, and thereby reduces the overall cost associated with. Zhang et al AMB Expr (2020) 10:19 the enzymatic bioconversion processes (Jørgensen and Pinelo 2017). Different strategies including enzyme readsorption on fresh solid substrate, membrane separation and enzyme immobilization have been developed to overcome, at least in part, these problems
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