Abstract
Diallate [S-(2,3-dichloroallyl)diisopropylthiocarbamate] incorporated into sand significantly inhibited sorghum ( Sorghum bicolor (L.) Moench cv Funks G 522DR) growth of 14-day-old seed lings. Inhibition was competitively reversed by exogenous giberellic acid (GA 3) (0.1 and 10 ppmw). Diallate inhibited gibberellin (GA) precursor biosynthesis in a cell-free enzyme preparation from unruptured, etiolated sorghum coleoptiles. Diallate (10 μ M) inhibited kaurene oxidation 40% with a 2.7 × increase in kauren-ol and a 50% decrease in kaurenoic acid. The GA biosynthesis inhibition correlates with symptom phenology and field use application concentrations. Geranylgeranyl pyrophosphate accumulated 5 × at 0.1 μ M diallate concentrations but concomitant kaurene concentration decreases did not occur. At 10 μ M diallate, kaurene synthetase was inhibited 33%.
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