Abstract
9533 Background: The identification of malignant cells in effusions by conventional cytology is hampered by its limited sensitivity. Previous studies show that the MAGE genes families are only expressed in the malignant cells and not expressed in normal tissues except testis and placenta. The aim of this study was to improve tumor cell detection in effusions by reverse-transcriptase polymerase chain reaction (RT-PCR) for MAGE families expression. Methods: A total of 22 thoracic effusions from patients with lung cancer and 18 thoracic effusions from patients without an evidence of malignancy were included in this study. All effusion specimens were evaluated in parallel by cytology, RT-PCR for MAGE families expression and pleural biopsy under video assisted thoracoscopy. To improve the sensitivity of RT-PCR, we combined the results of RT-PCR for MAGE-1, 2, 3 and 4 expressions. Anyone of them expressed in the effusion was recorded as MAGE positive. Results: Tumor cell detection by cytology, RT-PCR for MAGE e...
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