Abstract
Background Sepsis is a life-threatening condition and a systemic inflammatory response syndrome (SIRS) driven by infection. This study aimed at investigating the expression of microRNA-103 (miR-103) in sepsis patients, evaluating its diagnostic value, and exploring the regulatory effect of miR-103 on LPS-induced inflammation in monocytes. Methods Expression of miR-103 was measured using quantitative real-time PCR. A receiver operating characteristics curve was plotted to evaluate the diagnostic vale of miR-103. Serum and cell supernatant levels of proinflammatory cytokines were analyzed using ELISA. The interaction between miR-103 and Toll-like receptors 4 (TLR4) was analyzed using luciferase reporter assay. The effect of miR-103 on inflammation was examined in LPS-treated monocytes. Results Serum expression of miR-103 was decreased in noninfectious SIRS and sepsis patients compared with healthy controls, and the lowest expression value was observed in sepsis patients (all P < 0.05). Serum levels of miR-103 have considerable diagnostic accuracy in distinguishing sepsis patients from SIRS patients and healthy controls. A negative correlation was found between miR-103 and inflammatory responses in sepsis patients. TLR4 was demonstrated to be a direct target of miR-103 and was negatively regulated by miR-103 in monocytes. The promoted inflammatory responses by LPS in monocytes were reversed by the overexpression of miR-103. Conclusion All the data revealed that serum decreased miR-103 in sepsis patients serves as a promising noninvasive diagnostic biomarker and may be involved in the pathogenesis of sepsis by regulating inflammatory responses via targeting TLR4.
Highlights
Sepsis is a kind of systemic inflammatory response syndrome (SIRS) driven by infection [1]
The disease severity of the sepsis patients was defined by the sepsis-related organ failure assessment (SOFA) score [19] and the acute physiology and chronic health evaluation (APACHE) II score [20] obtained on day 1 after intensive care units (ICUs) admission
This study summarized the clinicopathological features of the sepsis patients, including body mass index (BMI), white blood cell (WBC), C-reactive protein (CRP), PCT, APACHE II score, and SOFA score
Summary
Sepsis is a kind of systemic inflammatory response syndrome (SIRS) driven by infection [1]. The gold standard for sepsis diagnosis is blood microbiological culture analysis, but this method spends more time to get the examination results than other molecular biomarkers, such as procalcitonin (PCT) and C-reactive protein (CRP) [5]. These frequently used molecules lack specificity in distinguishing sepsis cases from noninfectious diseases [6]. All the data revealed that serum decreased miR-103 in sepsis patients serves as a promising noninvasive diagnostic biomarker and may be involved in the pathogenesis of sepsis by regulating inflammatory responses via targeting TLR4
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