Abstract
Objective To estimate the value of immunohistochemical staining for C3d and C4d in paraffin-embedded tissue sections for the auxiliary diagnosis of bullous pemphigoid (BP). Methods Paraffin-embedded tissue sections from 20 patients with BP served as the experiment group, and those from lesions of 9 patients with familial benign pemphigus and 4 patients with epidermolysis bullosa as well as from normal skin served as the controls. Immunohistochemical SP method was used to measure the expressions of C3d and C4d in these paraffin sections. Results The deposition of C3d and C4d was observed in the basement membrane at the dermo-epidermal junction in 95% (19/20) of BP tissue sections, but undetected in any of the familial benign pemphigus or epidermolysis bullosa tissue sections. Conclusion Immunohistochemical staining for C3d and C4d in paraffin-embedded tissue sections may serve as an auxiliary method for the diagnosis of BP. Key words: Pemphigoid, bullous; Paraffin embedding; Microtomy; Immunohistochemistry; Complement C3d; Complement C4d
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