Diagnostic Usefulness of Inflammatory Markers in Acute Cellular Rejection After Heart Transplantation

Abstract

Background Acute cellular rejection (ACR) affects early morbidity and mortality after heart transplantation. The diagnostic technique of choice is endomyocardial biopsy. Our aim was to evaluate the diagnostic usefulness of inflammatory markers as a noninvasive method to monitor cellular rejection. Material and Methods We prospectively analyzed 73 cardiac transplant patients by determining the serum levels of protein fibrinogen (fgpro), functional fibrinogen (fgfun), C-reactive protein (CRP), and sialic acid (SA) coinciding with an endomyocardial biopsy (5.1 revisions/patient). The statistical methods were χ 2, Student’s t-test, and ROC curves. Results Of the 373 controls, significant rejection was detected in 19%. Analysis of the relationship between ACR and the markers showed significantly elevated levels of fgpro (345 ± 90 versus 307 ± 74 mg/dL; P = .03), fgfun (361 ± 101 versus 318 ± 89 mg/dL; P = .04), and SA (74 ± 22 versus 66 ± 15 mg/dL; P = .02), but not CRP (19 ± 29 versus 10 ± 21 mg/dL; P = .07). SA displayed a better diagnostic utility (area under the curve 0.7; P < .01), 35% sensitivity, 85% specificity, and 82% negative predictive value for a cutoff point of 80 mg/dL. Conclusions Among the inflammatory markers increased in ACR, SA was the most useful noninvasive tool for screening.