Diagnostic Study on NSP5 of Human Rotavirus in Najaf Governorate

Abstract

Objectives: Human rotavirus is the common cause of diarrhea-related illness and death among infants and young children, can lead to severe and sometimes lethal dehydration . Methodology: This study deals with the prevalence of rotavirus during a period extending of March to December 2014 . A total of 120 samples of diarrheal samples from patients up to 5 years age, who were admitted for medically care unit in the hospital of pediatrics and maternity of Al-Najaf governorate were investigated by latex agglutination, based on specific monoclonal antibodies directed against VP4 and VP7 of human rotavirus then the detection by PCR technique and isolation of the virus on these culture. Results: Rota virus was detected in 80 cases (66.7%) of the total cases investigated by latex agglutination test, while PCR positivity was recorded in 85 cases (70.8%). Rota virus infection was also detected in healthy children who were involved as control group; 1/50(2%) detected by Latex agglutination assay versus 3/50 (6%) detected by NSP5 gene PCR assay. From the total of ten isolates that selected to propagate via cell culture using rhabdomyosarcoma (RD) cells, confirmation of the detection was accomplished by 8 isolates (80 %). Conclusions: We concluded that polymerase chain reaction is sensitive and specific technique for the detection of NSP5 coding gene and RD cell line is successful for the propagation of rotavirus. Recommendations: we recommended to achieve future therapeutic studies depending on this study conclusions.Key words: RD cell culture, rotavirus NSP5, real-time PCR.