Abstract
The present study explored the clinical value of plasma microRNA-21 as a novel biomarker for early prediction of stable and unstable angina patients and its relationship with aging. A total of 255 participants, 123 patients with chronic stable angina, 82 patients with unstable angina, and 50 healthy subjects, were included in our study. Stable coronary and unstable coronary patients were confirmed following AHA/ACC clinical protocols. Total RNA was extracted from plasma by using miRNA-based TRIzol reagent. Plasma miR-21 expression levels were determined by real-time polymerase chain reaction. To evaluate the diagnosis accuracy, the receiver operating characteristic (ROC) curves were used. Plasma microRNA-21 concentration levels were significantly elevated in stable and unstable angina patients as compared with control subjects (P < 0.001). The area under the ROC curves of circulating microRNA-21 was accurately distinguished in stable angina patients (AUC 0.921) and unstable angina patients (AUC 0.944) from healthy subjects. MicroRNA-21 expression gradually elevated with increasing aging in all the populations. Moreover, the current study also demonstrated that the expression of plasma miR-21 levels was significantly associated with different age groups within healthy subjects and stable and unstable angina patients (P < 0.001). This research finding suggested that plasma microRNA-21 may be considered as a suitable new biomarker for early detection of stable and unstable angina patients, and it has a strong correlation with aging.
Highlights
Coronary artery disease (CAD) is a major public health problem and remains the leading cause of sudden cardiac death (SCD) all over the world
Almost 50% of these chest pain cases were of cardiac origin; either stable angina pectoris, unstable angina, acute coronary syndrome, or acute myocardial infarction (AMI) [1]
It has been demonstrated that expressions of several specific circulating microRNA levels were significantly altered in stable angina pectoris patients, unstable coronary artery disease, and acute coronary syndrome
Summary
Coronary artery disease (CAD) is a major public health problem and remains the leading cause of sudden cardiac death (SCD) all over the world. It has been demonstrated that expressions of several specific circulating microRNA levels were significantly altered in stable angina pectoris patients (miR-19a, miR-133a, miR-149, and miR-208a), unstable coronary artery disease (miR-423, miR-424, and miR-765), and acute coronary syndrome (miR-1, miR-92a, miR-134-5p, and miR-183-5p). Erefore, the current study investigated the diagnostic potential of circulating microRNA-21 for early detection of stable angina and unstable angina patients and the relationship between plasma miR-21 and aging.
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