Abstract
Objectives. To evaluate the role of angiogenesis tumor marker CD31 in the detection of precancerous and cancerous cervical lesions and to compare its efficacy with colposcopy and histopathology. Materials and Methods. 230 patients with a suspicious looking cervix and an abnormal Pap smear attending the Outpatient Department of Obstetrics and Gynaecology of GSVM Medical College were subjected to a colposcopic examination. 180 patients with suspected colposcopic findings were subjected to a colposcopic directed biopsy. Biopsy tissues were sent for histopathological examination out of which 50 biopsied samples were sent for immunostaining of CD-31. Statistical analysis was done. Results. Comparison of microvessel density (MVD) count by haematoxylin and eosin staining (HE) and immunostaining of CD31 in preinvasive group were 4.012 ± 2.57 and 5.44 ± 2.21, respectively, and in invasive group were 9.18 ± 2.32 and 12.82 ± 4.07, respectively, which showed that MVD was higher by CD31 both in preinvasive and invasive group, and it was statistically significant. Conclusion. Angiogenesis is a marker of tumor progression, and CD31 fixes up vessel better as compared to HE, so aggressiveness of the tumor can be better predicted by MVD-CD31 as compared to MVD-HE.
Highlights
Worldwide cervical cancer is the most common gynaecological malignancy and the third most common frequently diagnosed cancer in female after breast and colorectal cancer
Intensity of tumor angiogenesis is supposed to be reflected by intratumoral microvessel density (MVD), but it is difficult to assess revascularization by normal microscopy; various antibodies members for endothelial cells have been used to demarcate the area of vascularity within the tumor out of which CD-31 has the best documented specificity for endothelial cells
This was a prospective study with total number of 250 patients who attended the Outpatient Department of Obstetrics and Gynaecology of GSVM Medical College, Kanpur, from January 2007 to October 2008, out of which 230 patients with suspicious looking cervix and abnormal pap smear were subjected to colposcopy between the 8th and the 12th days of the menstrual cycle. 180 patients with suspected colposcopic findings were subjected to a colposcopy directed biopsy, and tissues were sent for histopathological examination in the Department of Pathology, GSVM Medical College Kanpur
Summary
Worldwide cervical cancer is the most common gynaecological malignancy and the third most common frequently diagnosed cancer in female after breast and colorectal cancer. It is the commonest cause of gynaecological cancer death and responsible for about 190,000 deaths every year [1]. CD-31 is a cluster of differentiation molecule with molecular weight of 130–140 Kilodaltons belonging to the immunoglobuin supergene family. It plays a major role in tissue regeneration and is normally found on endothelial cells, platelets, and on macrophages along with the expression on vascular tumors
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