Abstract
Inula cappa (Buch.-Ham. ex D. Don) DC has been used in traditional Chinese medicine to treat malaria, dysentery, and hepatitis. Previous studies have shown that chlorogenic acid is the effective ingredient of plants in this family. And the research of the chlorogenic acid in Inula cappa will help to further improve the effective resource utilization rate of this plant. Therefore, it is necessary to establish an accurate method to characterize the chlorogenic acid components in Inula cappa. In this study, a simple, fast, and sensitive UHPLC-Q-Exactive Orbitrap mass spectrometry method was established, which can simultaneously analyze known and unknown ingredients in a short time (within 30 minutes) in Inula cappa. According to the diagnosis fragmentation ions, retention time, and bibliography, 68 chlorogenic acid derivatives were identified in Inula cappa. The results of this experiment lay the foundation for the active substances and quality control of Inula cappa and provide a theoretical basis for whether Inula cappa can be an alternative to the endangered wild medicinal materials of the same family.
Highlights
Traditional Chinese medicine (TCM) has been applied for thousands of years in China and its surrounding areas
It is still unclear which of the ingredients of TCM are effective for the treatment of diseases because they are a complex mixture of hundreds of different chemical components
A simple, fast, and sensitive UHPLC-QExactive Orbitrap mass spectrometry method was established, based on accurate mass, which can simultaneously analyze the known and unknown components in Inula cappa in a short time
Summary
Jie Peng ,1 Jing Xie ,2 Silin Shi ,1 Lilan Luo ,1 Kailin Li ,1 Pei Xiong ,1 and Wei Cai 1,3. Previous studies have shown that chlorogenic acid is the effective ingredient of plants in this family. The research of the chlorogenic acid in Inula cappa will help to further improve the effective resource utilization rate of this plant. Erefore, it is necessary to establish an accurate method to characterize the chlorogenic acid components in Inula cappa. A simple, fast, and sensitive UHPLC-Q-Exactive Orbitrap mass spectrometry method was established, which can simultaneously analyze known and unknown ingredients in a short time (within 30 minutes) in Inula cappa. According to the diagnosis fragmentation ions, retention time, and bibliography, 68 chlorogenic acid derivatives were identified in Inula cappa. According to the diagnosis fragmentation ions, retention time, and bibliography, 68 chlorogenic acid derivatives were identified in Inula cappa. e results of this experiment lay the foundation for the active substances and quality control of Inula cappa and provide a theoretical basis for whether Inula cappa can be an alternative to the endangered wild medicinal materials of the same family
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