Abstract
背景与目的肺癌是临床上最为常见的恶性肿瘤,晚期患者预后差,5年生存率低,因此早期诊断成为提高患者预后的关键。近年来随着分子生物学技术的发展,一些关键驱动基因的异常修饰如甲基化成为肺癌早期诊断的重要方法。本研究旨在采用循证医学方法量化评价矮小同源盒基因(short stature homeobox 2, SHOX2)启动子区域异常高甲基化诊断肺癌价值。方法系统检索MEDLINE、EMBASE、Ovid、Web of Science、中国期刊全文数据库(CNKI)中涉及SHOX2基因启动子区域甲基化与肺癌关系的相关文献,并根据纳入与排除标准进行文献筛选。提取原始研究中SHOX2启动子甲基化相关数据,计算以SHOX2启动子甲基化为参考诊断肺癌的敏感性、特异性及受试者工作特征曲线(receiver operating characteristic curve, ROC)下面积。结果最终纳入本次meta分析的文献13篇,各项研究间存在明显的统计学异质性(P < 0.05),数据合并均采用随机效应模型。SHOX2基因启动子甲基化诊断肺癌的敏感性为0.75(95%CI: 0.74-0.77)、特异性为0.89(95%CI: 0.88-0.91);阳性预测值为6.75(4.56-9.99),阴性预测值为0.36(0.25-0.52);诊断优势比为23.16(11.34-47.31),ROC曲线下面积为0.9。结论SHOX2基因启动子高甲基化在肺癌患者血清、支气管灌洗液和胸腔积液中发生率均较高,可作为辅助诊断肺癌的生物学标志物。
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Disclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.