Diagnostic Difficulties withPlasmodium knowlesiInfection in Humans

Erma Sulistyaningsih,Thomas Löscher,Nicole Berens-Riha,Loeki Enggar Fitri

doi:10.3201/eid1606.100022

Abstract

Diagnostic Difficulties with<i>Plasmodium knowlesi</i>Infection in Humans

Highlights

To the Editor: Studies conducted in Malaysia have raised questions about Plasmodium knowlesi as the fifth human pathogenic malaria parasite [1,2]; additional cases of P. knowlesi malaria have subsequently been reported from other Asian countries [3,4,5]
Sulistyanisih, unpub. data), during December 2008–February 2009, blood samples from 22 gold miners with uncomplicated malaria were collected in South Kalimantan Province in Indonesia
DNA was extracted and species were identified by nested PCR by using Plasmodium genus- and speciesspecific primers derived from the small subunit RNA gene described elsewhere [1]

Summary

Introduction

To the Editor: Studies conducted in Malaysia have raised questions about Plasmodium knowlesi as the fifth human pathogenic malaria parasite [1,2]; additional cases of P. knowlesi malaria have subsequently been reported from other Asian countries [3,4,5]. Singh et al has designed a nested PCR assay for identification of P. knowlesi infections [1]. Data), during December 2008–February 2009, blood samples from 22 gold miners with uncomplicated malaria were collected in South Kalimantan Province in Indonesia. DNA was extracted and species were identified by nested PCR by using Plasmodium genus- and speciesspecific primers derived from the small subunit RNA gene described elsewhere [1].

Results

Conclusion