Diagnostic and Epidemiologic Study of Human Brucellosis at Al-Najaf province

Abstract

Background: Maltese fever is one of the dangerous occupational diseases for dairy producers, processor and staff cattle slaughterhouse, veterinarians and laboratory personnel. Infection accompanies a wide range of clinical symptoms that require the use of effective diagnostic tests. The choice of the epidemiological diagnosis in the region and the decision of the infection depends on the size of the infestation., Clinical information provided by the patient must include the geographical area, work type Was traveling to rural areas or affected countries this disease Is a family member with the disease, treatment he takes the patient what is positive and negative impact on the patient. Germ Malta fever tend to tissue rich in blood vessels Aims of study: The study aims to evaluate the epidemiological aspects of the population according to gender and age. And the study of the property and the sensitivity of the two tests, the first is the use of a conventional test (Rose Bengal), and modern molecular technique (RT- PCR) and compare the two results. Methodology: Type of study is a diagnostic study includes 41 blood samples of patients with suspected Malta fever, in both gender diagnosed questionable, the ages were between 14-45 years. Patients were attending to the province of Al-Najaf Al-Ashraf of rural area surrounding the governorate and the center of the province, and attend private clinic on January to December 2015 in order to evaluate serum infection of Malta carried out using serological testing and technology of molecular test method Results: A total of 41 patients with suspected brucellosis had been included in this study. The most frequent age group was more than 35(29.3%) years. The percentage to an urban cases were (58.54%) and the percent of rural area. Brucellosis, variations during one year shows positive predictive value=50%, Negative predictive value=95.2% and Accuracy =73.1 % of diagnosis of serological compared to Real time, PCR proportion of 41 patients. High sensitivity (90.9 %) has detection of Brucellosis by Real Time PCR, and the Specificity=66.7%. Conclusion: Estimated incidence in most regions injury (rural areas) may be less compared to the younger population. Medical safety is very important in the laboratory. Organization of the CDC classifies Iraq in high incidence areas with Malta fever. Recommendations: Avoid unpasteurized hand milk products and fresh milk which is not boiled. Clinical laboratories must be under the terms of health safety when isolate the pathogen. And early treatment of the disease at the onset of clinical signs of the disease eliminates protect against chronic doubled.