Diagnostic algorithm for comorbidity of babesiosis and dirofilariasis in dogs, taking into account the correlation of redox homeostasis and morphofunctional disorders of the hepatorenal and cardiopulmonary systems

Tatyana Ushakova,Anna Fomina,Tatyana Derezina,Ivan Kapelist,D Rudoy,N Ugrekhelidze,A Olshevskaya

doi:10.1051/e3sconf/202127302022

Abstract

Activation of lipid peroxidation due to a disorder of redox homeostasis in dogs with comorbidity of babesiosis and dirofilariasis causes the involvement of not only the components of the hepatorenal system, but also the cardiopulmonary system in the pathological process against the background of the development of intoxication and hypoxic phenomena in hepatocytes and cardiomyocytes. As a result of the conducted biochemical studies of the blood of sick animals, it was found that the degree of redox homeostasis disorders directly correlates with the nature of morphofunctional disorders of the hepatorenal and cardiopulmonary systems, and the degree of these changes in the systems has an inverse correlation and is determined by the release of cytosolic enzymes into the blood. The involvement of the components of the hepatobiliary system in the pathological process is accompanied by a disorder of protein, carbohydrate, lipid and pigment metabolism.

Highlights

As a result of clinical studies of sick animals, it was found that the manifestation of morphofunctional disorders of the hepatorenal and cardiopulmonary system in comorbidity of babesiosis and dirofilariasis in dogs was characterized by the development of signs of anxiety, general weakness, apathy, dyspnea during exercise, tachycardia, tachinpoe, anorexia and polydipsia.The development of pyretic fever
Morphofunctional disorders of the components of the hepatobiliary system were accompanied by a disorder of protein, carbohydrate, lipid and pigment metabolism, an increase in the GGT/alanine aminotransferase (ALT) and GGT/alkaline phosphatase (ALP) coefficient, a decrease in the level of cholinesterase (CHS), which confirmed the involvement of the liver parenchyma in the pathological process and massive death of hepatocytes due to parasitization of Babesiacanis
With the comorbidity of babesiosis and dirofilariasis in dogs, there is a direct correlation between the level of morphofunctional disorders of the hepatorenal system and the nature of redox homeostasis disorders, which appears as a disorder of the metabolic activity of the liver, an increase in the catalytic activity of serum enzymes due to the activation of lipid peroxidation processes, as well as a disorder of nitrogenous exchange

Summary

Introduction

The aim of our research was to study the nature of the correlation between redox homeostasis and morphofunctional disorders of the cardiopulmonary and hepatorenal systems in dogs with an associative course of babesiosis and dirofilariasis. To achieve this goal, we set the following tasks: to study the clinical status, biochemical blood parameters, data of ultrasonographic studies of the hepatobiliary system in dogs with mixed invasion of dirofilariasis - babesiosis.

Objectives

Results

Discussion

Conclusion