Abstract
Although the rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. We compared the diagnostic performance of a “sweeping method” for H. pylori detection with the conventional biopsy sampling method in atrophic gastric conditions which can reduce RUT accuracy. This prospective study included 279 patients who underwent upper endoscopy to determine the presence of H. pylori infection. Gastric mucosa of both the antrum and the corpus were swabbed, and we named this method the “sweeping method”. Biopsy sampling for the conventional method, histologic evaluation, and polymerase chain reaction were performed at the same time. The sensitivity, specificity, and accuracy of the sweeping method were 0.941, 0.826, and 0.903, respectively, compared to 0.685, 0.859, and 0.742, respectively, for the conventional biopsy method. The area under the receiver operating curve for the sweeping method was 0.884 versus 0.772 for the conventional method (P < 0.001). The sweeping method had a faster detection time than the conventional method. Compared to conventional biopsy sampling, the sweeping method with the RUT provided higher sensitivity and accuracy for the detection of H. pylori, with a faster detection time.
Highlights
The rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection
Our “sweeping method” consists of collecting as many H. pylori organisms as possible by absorbing the gastric mucus using swabs, without requiring direct sampling of the gastric tissue. We hypothesized that this sweeping method could provide a more accurate and faster detection of H. pylori infection than the conventional biopsy sampling method with the RUT, with better safety profile in lowering the risk for adverse events. This prospective study aimed to evaluate the diagnostic performance of our sweeping method against the conventional biopsy sampling method, for the detection of H. pylori infection using RUT
We developed a new method using a commercially available RUT kit that overcomes the drawbacks of the conventional biopsy sampling method
Summary
The rapid urease test (RUT) is a simple method for detecting Helicobacter pylori (H. pylori) infection, it requires sufficient biopsy samples and its sensitivity varies depending on the site and condition of H. pylori infection. The rapid urease test (RUT) is the commonly used invasive method for H. pylori detection, where tissue samples from the gastric mucosa are placed into a commercially available analysis kit, with results, interpreted from a change in color, requiring minutes to hours. We hypothesized that this sweeping method could provide a more accurate and faster detection of H. pylori infection than the conventional biopsy sampling method with the RUT, with better safety profile in lowering the risk for adverse events.
Sign-in/Register to view highlights & summary Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
