Abstract
The colorectal cancer is a serious health problem. The diagnosis of the disease mostly relies on an invasive procedure. A non-invasive diagnostic test such as an immunoassay, may facilitate diagnosis of colorectal cancer. The purpose of the study was to evaluate the use of antibodies against Fusobacterium nucleatum in the diagnosis of colorectal cancer (CRC). Totally 78 patients in three groups were included in the study. F. nucleatum in the tissues was detected using quantitative polymerase chain reaction assay. F. nucleatum IgA and IgG were measured using enzyme linked immunosorbent assay. F. nucleatum was detected in 86.7% and 73.1% cases of CRC and precancerous-benign colon disease (P-BCD), respectively. The OD values from F. nucleatum IgA and IgG ELISA tests were higher in CRC group compared with healthy individuals. The sensitivity of IgA ELISA test varied between 31.8 and 95.5% depending on the chosen cut-off values. The positivity rate of antibodies in patients with high amount of F. nucleatum in tissue was significantly greater than in the negative group. The F. nucleatum IgA and IgG antibodies in CRC were higher than the ones in healthy controls but the discriminative ability of the ELISA test was not adequate to be considered as a diagnostic tool.
Highlights
Colorectal cancer (CRC), an important threat to human health, is one of the most common cancer in the w orld[1]
A total of 78 patients were placed in three groups (22 colorectal cancer (CRC), 35 precancerousbenign colon disease (P-BCD) and 21 Healthy controls (HC))
In 18 (81.8%) CRC cases, tumor was on distal colon and in 4 (18.2%) CRC cases, tumor was on proximal colon
Summary
Colorectal cancer (CRC), an important threat to human health, is one of the most common cancer in the w orld[1]. Human gut hosts highly diverse and complex microbial community including virus, fungi and b acteria[2]. There is a small amount of F. nucleatum found in the gut microbiota, it is supported that it may cause infection and cancer in the colon tissue[4]. The immune system can produce antibodies to keep host from invasion of microorganisms in colon. Those antibodies are sometimes extremely useful for the diagnosis of diseases especially when the pathogen organism cannot cultivate. Antibody tests are important laboratory tools for the diagnosis of infectious disease because they are reliable and easy to use. The purpose of the study was to evaluate the use of antibodies against F. nucleatum in the diagnosis of CRC. The reference tests were a colonoscopic examination and pathological evaluation of biopsy material
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