Abstract
SUMMARY Ehrlichia canis was demonstrated by direct immunofluorescence in buffy coat and other tissue smears using fluorescein conjugates prepared from the sera of infected dogs. The antigenic forms of E. canis demonstrated by immunofluorescence were identical with those demonstrated by Giemsa’s stain. Low magnification scanning of immunofluorescent preparations was quicker and more sensitive than the examination of similar preparations stained with Giemsa. E. canis could also be demonstrated by staining with acridine orange. Tests for infcctivity in dogs were more sensitive than any of these 3 visual methods. Sera and smears from dogs infected with strains of E. canis isolated from widely separated areas of the world were examined by immunofluorescence and all appeared to be identical antigenically.
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