Abstract
Objectives To identify an effective bioassay for human hepatitis B virus (HBV) using voltammetric sensor. Study design Electrode was prepared with bovine IgG immobilized onto a DNA-linked carbon nanotube electrode (BIDCE). Analytical BIDCE conditions were optimized through square wave (SW) stripping and cyclic voltammetry (CV). Results Optimum parameters of 75 Hz SW frequency, 0.1 V SW amplitude, −1.3 V SW accumulation potential, 100 s accumulation time, and 3.0 mV SW increment potential were obtained. Within working ranges of 0.035–0.242 mg/mL anti-bovine IgG, the relative standard deviation of 0.2 mL HBV was observed to be 0.04 ( n = 4). Conclusions Diagnostic application was performed through direct assay of HBV in non-treated human blood.
Talk to us
Join us for a 30 min session where you can share your feedback and ask us any queries you have
Schedule a callDisclaimer: All third-party content on this website/platform is and will remain the property of their respective owners and is provided on "as is" basis without any warranties, express or implied. Use of third-party content does not indicate any affiliation, sponsorship with or endorsement by them. Any references to third-party content is to identify the corresponding services and shall be considered fair use under The CopyrightLaw.
