Diagnosis of Gallibacterium Anatis in Layers: First Report in Turkey

S Yaman,O Sahan Yapicier

doi:10.1590/1806-9061-2019-1019

S Yaman, O Sahan Yapicier

Open Access

https://doi.org/10.1590/1806-9061-2019-1019

Copy DOI

Abstract

ABSTRACT Gallibacterium anatis, a member of the Pasteurellaceae family, leads to decrease in egg-production, animal welfare and increase in mortality. This study aimed to diagnose G. Anatis, which caused economic losses in laying hens by using conventional and molecular techniques. In this study, G. anatis was examined from a total of 200 dead chicken tissues (heart, liver, lung, spleen and trachea) in laying hen farms that observed a decrease in egg production with respiratory system infection. Conventional methods based on colony morphology, sugar fermentation tests and hemolytic properties and molecular conformation using 16S rRNA-23S rRNA specific primers were performed to identify G. anatis. G. anatis was isolated in 20 (10%) of the examined samples and isolates were confirmed by conventional PCR. A total of 11 (2.2%) positivity was obtained as isolates were the result of PCR performed on tissues and organs directly. As a result, the presence of G. anatis was detected for the first time in Turkey by this study. It was thought that G. anatis may have a role in egg production losses due to respiratory tract infection in poultry and this situation may be a guide for poultry clinicians and microbiologists.

Highlights

Major health problems of the poultry industry have certain effects on egg production
G. anatis is an infectious agent that has been isolated from broiler and egg-laying chickens with salpingitis and peritonitis in various countries around the world in recent years, and is associated with economic losses due to the resulting decline in egg yield (Bojesen et al, 2003; Elbestawy et al, 2018)
It is thought that the reason why G. anatis has not been detected to date is due to the similarity of the symptoms of this infection to that of various respiratory tract infections, and to the symptoms of fowl cholera, and the fact that the precise taxonomic classification of the bacteria was not established until 2003

Summary

Introduction

Major health problems of the poultry industry have certain effects on egg production. Gallibacterium anatis (G. anatis) has been known to be a part of the normal microflora of the lower genital and upper respiratory tract (Bojesen et al, 2004; Jones et al, 2013; Lawal et al, 2018; Persson & Bojesen 2015; Rzewuska et al, 2007). In recent years, decreased egg production associated with oophoritis, follicule degeneration, salpingitis, respiratory system disorders and increased mortality in commercial layers has accelatered interest in G. anatis infections (Alispahic et al, 2011; Bisgaard et al, 2009; Bager et al, 2013; Bojesen et al, 2003; Bojesen, 2003; Sing, 2016; Chaveza et al, 2017; Johnson et al, 2013; Paudel et al, 2014). The epidemiology and bacteria-host interactions of Gallibacterium spp. are little understood due to a lack of published literature and previous uncertainty with regard to the identification of bacteria representing this genus (Bisgaard, 1993)

Objectives

Methods

Results

Conclusion