Abstract
The first investigation ordered on clinical suspicion of dengue is complete blood count. The classical dengue triad includes increased hematocrit, atypical plasmacytoidlymphocytosis and thrombocytopenia [1].Hemoconcentration (hematocrit elevated > 45% or by > 20% from previous value) is diagnostic of Dengue Hemorrhagic Fever. Further confirmation is usually made with Dengue Antigen test. During early stages of the disease around 4-5 days of fever onset, virus isolation, nucleic acid or antigen detection are used to diagnose the virus from serum, plasma or tissues. However serology remains the method of choice during convalescence. Other tests include antibody titre for dengue virus types, Polymerase chain reaction (PCR), Liver function tests, Serum protein & albumin levels & Coagulation panel.
Highlights
1) Fourfold change in immunoglobulin G (IgG) or IgM antibody titre to 1 or more dengue virus antigens [2]
2) Tissue dengue virus antigen assay by immunomolecular beacon probes [6]
The isolated viruses are detectedby indirect immunofluorescence using monoclonal antibodies against all four serotypes [7]
Summary
1) Fourfold change in immunoglobulin G (IgG) or IgM antibody titre to 1 or more dengue virus antigens [2]. Lab criteria for dengue diagnosis include any one amplification) assay and RT-LAMP are recent of the following 4 tests– Assay is an isothermal RNA-specific amplification assay which includes an initial reverse transcription, RNA amplification & detection by electro-chemiluminescence or fluorescent-labelled 2) Tissue dengue virus antigen assay by immunomolecular beacon probes [6].
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