Abstract
ABSTRACT: Blackleg, an acute myonecrosis caused by Clostridium chauvoei, is usually underdiagnosed since the rapid transport of adequate samples for laboratory testing is difficult. This study tested a direct polymerase chain reaction (PCR) technique using common filter paper impregnated with cattle tissue samples obtained from animals suspected with blackleg. Twenty-five samples, belonging to eleven animals from Rio Grande do Sul State, Brazil, were analyzed. The direct PCR technique identified eight positive animals corroborating with results from microbiological culture. Skeletal muscle was the most common tissue type used in this study and when the animal was positive the pathogen was always detected in this tissue. Storage time of the impregnated filter paper at room temperature did not prove to be a limiting factor for the quality of the results indicating that this procedure can be carried out in the field and samples be sent in regular mail. Our results suggested that direct PCR of common filter paper impregnated with cattle tissue is a practical and economical alternative for the diagnosis of blackleg.
Highlights
RESUMO: Carbúnculo sintomático, uma mionecrose aguda causada por Clostridium chauvoei, costuma ser subdiagnosticada, pois o transporte rápido de amostras adequadas para exames laboratoriais é complicado
An acute, non-contagious myonecrosis caused by Clostridium chauvoei, leads to significant losses in the beef cattle industry and is globally responsible for outbreaks and sudden deaths of well-conditioned, young cattle (QUINN et al, 2011)
A definitive diagnosis requires the detection of C. chauvoei in affected tissues and this is challenging since farmers have to send tissue samples, correctly and on time, to the laboratory and this is usually economically unfeasible (ABREU et al, 2017)
Summary
RESUMO: Carbúnculo sintomático, uma mionecrose aguda causada por Clostridium chauvoei, costuma ser subdiagnosticada, pois o transporte rápido de amostras adequadas para exames laboratoriais é complicado. When combined with the tissue-impregnated common filter paper samples it eliminates the need for time-consuming procedures associated with DNA extraction Detection of C. chauvoei was demonstrated using direct PCR from common filter paper impregnated with bovine liver inoculated with controlled amounts of C. chauvoei strain ATCC 10092 (FARIAS et al, 2012).
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