Diagnosis of avian salmonellosis using PCR kits of different manufacturers

Abstract

Relevance. The problem in timely detection of this infection is that poultry contaminated with salmonellae may not show clinical signs of the disease. Polymerase chain reaction in the diagnosis of avian salmonellosis is designed for direct detection and identification of pathogenic agents, especially in cases where their detection is difficult by other diagnostic methods. Advantages of PCR tests allow to successfully use this method in veterinary laboratory practice. The aim of this work is to conduct comparative tests of diagnostic PCR kits of different manufacturers in the diagnosis of avian salmonellosis.Methods. To conduct comparative studies of pathological material for the presence of the DNA of Salmonella spp., Salmonella enteritidis and Salmonella typhimurium by molecular biological method, three commercial kits of domestic and imported production for isolation and detection of Salmonella DNA by PCR were used. Determination of the possibility of using membrane glass fiber materials, in the form of cards for taking dry samples of pathogenic material, for subsequent PCR was carried out in comparative tests with parallel studies of biomaterial delivered to the laboratory in frozen form.Results. Comparative studies of pathological material for the presence of Salmonella spp. DNA using three commercial kits for the isolation and detection of Salmonella DNA by polymerase chain reaction, imported and domestic production were carried out. It was found that when comparing the results obtained using kits of different manufacturers for the presence of Salmonella spp. DNA, comparable results were observed. The study was performed within the framework of the state assignment No. FGUG-2022-0009 of (FSC VIEV).