Abstract
An immunofluorescent method for the diagnosis of African swine fever (ASF) is described. Diagnostic samples inoculated onto PK15 swine kidney cell line monolayers on coverslips, are acetone-fixed and stained with fluorescein isothiocyanate-conjugated ASF convalescent serum globulin at 24, 48 and 72 hours after inoculation. Specific fluorescence is detected in ASF infected cells as granular or globular inclusions. The method detected ASF virus in materials from some infected pigs which produced no haemadsorption at all, or only after two to three serial passages in swine buffy coat cultures. This test may provide a sensitive and rapid method for diagnosing ASF in chronic infection.
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