Diagnosis and prevalence of ovine pulmonary adenocarcinoma in lung tissues of naturally infected farm sheep

Ganesh G Sonawane,Bhupendra Nath Tripathi,Rajiv Kumar,Jyoti Kumar

doi:10.14202/vetworld.2016.365-370

Ganesh G Sonawane, Bhupendra Nath Tripathi + Show 2 more

Open Access

https://doi.org/10.14202/vetworld.2016.365-370

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Journal: Veterinary world	Publication Date: Apr 1, 2016
Citations: 39	License type: cc-by

Affiliation: Central Sheep and Wool Research Institute

Abstract

Aim:This study was aimed to detect ovine pulmonary adenocarcinoma (OPA) in sheep flocks affected with pulmonary disorders at organized farm.Materials and Methods:A total of 75 sheep died naturally were thoroughly examined for the lesions of OPA during necropsy. Tissue sections from affected portion of the lungs from each animal were collected aseptically and divided into two parts; one each for polymerase chain reaction (PCR) and another for histopathology.Results:On PCR examination of lung tissues, six sheep (8%) were found to be positive for JSRV. Two of them were 3-6 months of age and did not show clinical signs/gross lesions of OPA. Four adult sheep positive on PCR revealed characteristic lesions of OPA on gross and histopathological examination.Conclusion:In the absence of known specific antibody response to the infection with JSRV, there is no diagnostic serological test available. The PCR assay employed in this study on lung tissues, using primers based on the U3 region of the viral long terminal repeat for JSRV would be helpful in the screening of preclinical and clinical cases of OPA in sheep.

Highlights

In Rajasthan sheep and goats are generally reared through an extensive system of rearing and are one of the major sources of sustainable livelihood of rural poor and have great economic value in terms of meat, wool and milk
A total of 75 sheep died naturally were thoroughly examined for the lesions of Ovine pulmonary adenocarcinoma (OPA) during necropsy
In rest of the animals, macroscopic and microscopic findings in the lungs were related to various types of pneumonia (congestion/hemorrhage, edema, consolidation and Figure-1: Polymerase chain reaction (PCR) amplification of U3 gene fragment of Jaagsiekte resolved on 2.0% agarose gel electrophoresis: Lane 1 - Non-template polymerase chain reaction (PCR) control; Lane 2 - Amplification of JAG U3 gene; Lane 3 - Negative control and Lane 4: 100 bp plus DNA ladder (Fermentas, Cat SM0323)

Summary

Introduction

In Rajasthan sheep and goats are generally reared through an extensive system of rearing and are one of the major sources of sustainable livelihood of rural poor and have great economic value in terms of meat, wool and milk. Ovine pulmonary adenocarcinoma (OPA) has been identified in a wide variety of breeds in many countries around the world including India [2]. It is responsible for severe economic losses to the sheep industry in many sheep rearing countries and the subclinical form of the disease affects growth rate, carcass weight, and milk and wool production [3]. OPA is believed to be the most important disease that can affect international trade as determined by the Office International des Epizooties (OIE) [4].

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