Diagnosing the bioactive compounds in Iraqi garlic (Allium sativum) by GC-MS and HPLC

Abstract

This study including diagnosing the bioactive compounds releasing from an action of alliinase with alliin in the Iraqi garlic by GC-MS and HPLC technique. The bioactive compounds resulting from the garlic by GC-MS were heptane,2,4-dimethyl,cyclopropyl bromide, octane,5-ethyl-2-methyl, diallyl disulphide,trisulfide,di-2-propenyl,tetrasulfide,di-2-propenyl,3-vinyl-1,2-dithiacyclohex-4-ene,3-vinyl-1,2 dithiacyclo hex-5-ene and eicosane at different percentages through periods of time ranged between 5-30 minutes when following its activity by GC-MS. The best time for an activity was found at 30 minutes. Diallyl disulphide was the highest percent of 51.92% and tetrasulfide, di-2-propenyl at percent of 19.49%, as well as standard allicin and prepared allicin that is the main product of the enzymatic decomposition was diagnosed by HPLC technique of Iraqi garlic at the same periods with high concentration 39.244%.