Diacron reactive oxygen metabolites and biological antioxidant potential tests for patients with age-related macular degeneration

Toshiyuki Matsuura,Hiroki Kaneko,Keiko Kataoka,Rina Namba,Rei Shibata,Seina Ito,Hideyuki Shimizu,Taichi Tsunekawa,Hiroko Terasaki,Ayana Suzumura,Toyoaki Murohara,Yasuki Ito,Kei Takayama

doi:10.1186/s12886-020-01334-y

Toshiyuki Matsuura, Hiroki Kaneko + Show 11 more

Open Access

https://doi.org/10.1186/s12886-020-01334-y

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Abstract

BackgroundPreviously, we showed that serum malondialdehyde (MDA) was significantly higher in patients with neovascular age-related macular degeneration (nAMD) than in those without AMD. The Diacron reactive oxygen metabolites (d-ROMs) and biological antioxidant potential (BAP) tests are known markers of oxidative stress. The aim of this study was to use d-ROMs and BAP tests to evaluate changes in systemic oxidative stress in patients with nAMD.MethodsBlood serum samples were collected from 34 patients with nAMD (mean age: 76.5 ± 7.7 years; 22 men) and 20 control subjects (mean age: 62.9 ± 14.0 years; 10 men), and d-ROMs and BAP tests were examined.ResultsIn men, the mean level of d-ROMs for the nAMD patients was significantly higher than that for the controls (312.0 ± 52.4 vs. 275.1 ± 45.5 U.CARR, respectively; P < .05). There was a significant correlation between d-ROM level and CNV lesion area in the male nAMD group (r = .42, P = .05). There were no significant differences in mean BAP test results between the nAMD patients and controls for either sex (men: 2241 ± 549 vs. 2136 ± 246 μmol/L; women: 2263 ± 292 vs. 2335 ± 161 μmol/L).ConclusionThe d-ROMs test may provide a useful indicator of nAMD in men but not in women.

Highlights

We showed that serum malondialdehyde (MDA) was significantly higher in patients with neovascular age-related macular degeneration than in those without Age-related macular degeneration (AMD)
Advanced AMD is classified according to its pathophysiology as either neovascular AMD or geographic atrophy (GA) [3]. neovascular age-related macular degeneration (nAMD) is characterized by choroidal neovascularization (CNV) and an increase in intraretinal/subretinal fluid, which is strongly associated with the overexpression of vascular endothelial growth factor (VEGF)
Correlations between serum Diacron reactive oxygen metabolites (d-Reactive oxygen metabolites (ROM)) levels and nAMD patient characteristics We evaluated correlations between d-ROM levels and age, logarithm of the minimum angle of resolution (logMAR) best-corrected visual acuity (BCVA), and CNV area separately for the male and female nAMD patients

Summary

Introduction

We showed that serum malondialdehyde (MDA) was significantly higher in patients with neovascular age-related macular degeneration (nAMD) than in those without AMD. We showed that serum malondialdehyde (MDA), a marker of oxidative stress, was significantly higher in patients with nAMD than in those without AMD, and that there was a significant correlation between serum MDA levels and the area of CNV [12]. The Diacron reactive oxygen metabolites (d-ROMs) test for measuring reactive oxygen metabolites (ROMs) in blood has been used for various biological and medical purposes [13, 14]. This test uses the Free Radical Analytical System (Diacron International, Grosseto, Italy) [15,16,17]. Despite its power as an oxidant, Matsuura et al BMC Ophthalmology (2020) 20:56

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