Diabetic wound healing activity of Elaeagnus conferta Roxb. leaf ethanol extract

Abstract

High blood glucose level and gangrene wounds are becoming difficult to cure for the medical sciences nowadays. The goal of the current study is to assess the diabetic wound healing activity of Elaeagnus conferta leaf ethanol extract (LEE). The qualitative and quantitative phytochemical screening of the LEE revealed the presence of total phenols (42.20 ± 1.3 μg/gm) and flavonoids (45.23 ± 1.2 μg/gm). HR-LCMS analysis of the LEE revealed the presence of 27 phytoconstituents, among them Telmisartan was expressed with higher concentration. Alloxan monohydrate induced diabetic rats were administered with LEE of Elaeagnus conferta and the extract was locally applied to excision, incision and dead space wound models of diabetic rats. LEE at the concentration of (200 mg /kg. /bw.) significantly reduced the blood glucose levels when compared to the untreated diabetic group. In excision wound models on 21st day of treatment, the percentage of epithelization was increased to 99.86±0.08 and in the incision model, tensile strength of the wound was increased to 798.7±37.08gm as compared to Povidine iodine ointment. In slico molecular docking studies of the Telmisartan ligand with the glycogen synthase kinase- 3 protein resulted with 2 hydrogen bonds and 13 hydrophobic interactions indicating the suppression of wound enhancing protein. Present study authenticates the antidiabetic property and wound healing efficacy of E. conferta leaf ethanol extract.