Abstract
We investigated whether differences in mitochondrial genome play a role in anesthetic-induced preconditioning (APC) in the diabetic heart model with “swapped” mitochondria. These rats have identical nuclear genome, but some bear Wistar (T2DNmtWistar) while others bear Fawn Hooded Hypertensive (T2DNmtFHH) mitochondrial genome. The production of reactive oxygen species (ROS) and changes in flavoproteins (FPs) fluorescence were monitored from ventricular myocytes in the presence of 1 MAC isoflurane by laser scanning confocal microscopy. Opening of mitochondrial permeability transition pore (mPTP) was detected by tetramethylrhodamine dye. During isoflurane, ROS were increased by 165% and 107% in T2DNmtFHH and T2DNmtWistar cardiomyocytes, respectively. In addition, isoflurane induced oxidation of mitochondrial FPs in T2DNmtFHH by 94% and by 35% in T2DNmtWistar myocytes. Compared to respective controls, APC increased the mPTP opening time in T2DNmtWistar but not in T2DNmtFHH myocytes. Our findings suggest that mitochondrial genome-related differences in the function of respiratory chain complexes have an impact on anesthetic sensitivity of the mitochondria. This in part could explain that APC protection against mPTP opening is compromised in T2DNmtFHH but not in T2DNmtWistar cardiomyocytes. Supported by P01GM066730 (ZJB) from the NIH, Bethesda, MD.
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