Diabetes induced testicular dysfunction amelioration by ethyl acetate fraction of hydromethanolic extract of root of Musa paradisiaca L. in streptozotocin-induced diabetic rat

Abstract

ObjectiveTo investigate the diabetic therapeutic potentiality and antioxidative efficacy of ethyl acetate fraction of hydro-methanol (40:60) extract of root of Musa paradisiaca Lam. (Musaceae) in streptozotocin-induced diabetic rat. MethodsStreptozotocin-induced diabetic state was confirmed by decreased serum insulin level and carbohydrate metabolomics i.e. increased fasting blood glucose level, glycated hemoglobin level and diminished glycogen contents in liver and skeletal muscle. Reproductive homeostasis alteration in diabetes was evaluated by reproductive organo-somatic indices, sperm count, motility and histological analysis of testicular seminiferous tubule along with levels of serum testosterone, testicular cholesterol and seminal vesicular fructose assessment. Oxidative stress in primary and accessory sex organs, and in sperm pellet was assessed by measuring antioxidant enzyme activities along with quantification of free radicals products. Testicular pro-apoptotic Bax-α mRNA expression pattern was studied semi-quantitatively by PCR technique. Reverse phase HPLC fingerprinting was performed using methanol and acetonitrile as mobile phase. ResultsOral administration of ethyl acetate fraction at a dose of 20 mg/0.5 mL of distilled water/100 gm body weight twice daily to the diabetic rats for 28 days significantly recovered organo-somatic indices, protected reproductive activities, corrected oxidative stress markers and pro-apoptotic mRNA expression pattern, which were deviated in diabetes mellitus from control level without any type of toxicity. HPLC fingerprinting shows five completely resolved peaks at γ max 254 nm and 342 nm. ConclusionsIt has a promising antihyperglycaemic and antioxidative activity for curing diabetes induced reproductive disorders in streptozotocin-induced diabetic rat.