Abstract
An alternative method to 32P-postlabeling has been proposed for sensitive detection and quantitation of deoxyguanosine monophosphate - benzo[a]pyrenediol epoxide (dGMP-BPDE), a biomarker for human exposure to carcinogenic polycyclic aromatic hydrocarbons (PAH), using capillary electrophoresis with laser-induced fluorescence detection (CE-LIF). A modified CE-LIF instrument was adjusted to operate with a He/Cd UV laser (325 nm) for native fluorescence detection from BPDE adducts. The method was linear over three decades in concentration, with the detection limit of 2.5 × 10-9 mol L-1 at the signal-to-noise ratio of three after consecutive dilution of the dGMP-BPDE standard. At this level, recovery of 1 adduct per 10(7) normal nucleotides was possible. The RSD values for inter- and intra-day determination were better than 7% and recovery studies at three different levels yielded values around 50%. This method has been validated and for the first time applied to determination of dGMP-BPDE in blood samples from Brazilian rural workers, which were exposed to PAH in sugar-cane plantation harvesting and charcoal-production ovens.
Highlights
Human exposure to carcinogenic compounds such as polycyclic aromatic hydrocarbons (PAH) has been directly related to the incidence of cancer.[1]
The blood samples utilized were obtained from rural workers known to be occupationally exposed to PAH either during sugar-cane harvesting or in charcoal-production ovens
The concentration range of adduct was varied from 2.5×10-9 mol L-1 to 6.3×10-6 mol L-1 and the linearity was better than r = 0.998 over three decades in molar concentration
Summary
Human exposure to carcinogenic compounds such as polycyclic aromatic hydrocarbons (PAH) has been directly related to the incidence of cancer.[1] Such studies have related the development of cancer and other behavioral effects in population from highly polluted areas such as in iron foundry due the presence of the PAH. In Brazil, two typical cases in which workers are exposed to PAH are during the harvesting of the sugar-cane plantations and in charcoalproduction ovens. DGMP-BPDE DNA Adduct Investigation in Environmentally Exposed Rural Workers house-size brick ovens and are let burn not-to-completion. About half ton of charcoal is removed from the ovens by barely-dressed workers because of the excessive heat (hot weather plus the remaining heat from the oven). The whole environment is smoke and soot full, indicating that there is a high potential for PAH exposure.[3]
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