Abstract
Zinc finger proteins play a significant role in regulating plant metabolism and stress responses. Nevertheless, the role of CCCH-type zinc finger proteins in chrysanthemums remains unclear. In this study, DgC3H1 was isolated and cloned from chrysanthemum. The DgC3H1 predicted protein contained 282 amino acids and three CCCH zinc finger motifs (C-X5-14-C-X4-5-C-X3-H). Through subcellular localization, it was found that DgC3H1 is located in the nucleus and is a CCCH zinc finger transcription factor. DgC3H1 overexpression improved the low temperature resistance of chrysanthemum. The expression of endogenous C3H1 was downregulated under antisense inhibition, and the sensitivity of chrysanthemum to low temperature stress was increased. Under cold stress, the contents of proline and soluble sugar, and the activities of peroxidase and superoxide dismutase were enhanced in the DgC3H1 overexpression lines,and reduced in the antisense suppression lines. In addition, the low temperature stress response genes DgCOR413, DgDREBa, DgCSD1, and DgCSD2 were upregulated in the overexpression lines, and downregulated in the antisense suppression lines when compared to the wild type. These results indicate that DgC3H1 can improve the resistance of chrysanthemum to cold stress and can be selected as an excellent candidate gene for breeding cold-tolerant plants.
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