DGAT1 and ABCG2 polymorphism in Indian cattle (Bos indicus) and buffalo (Bubalus bubalis) breeds

Ramesh K Vijh,Bishnu P Mishra,Bina Mishra,St Bharani Kumar,Monika Sodhi,Madhu S Tantia

doi:10.1186/1746-6148-2-32

Ramesh K Vijh, Bishnu P Mishra + Show 4 more

Open Access

https://doi.org/10.1186/1746-6148-2-32

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Abstract

BackgroundIndian cattle (Bos indicus) and riverine buffalo (Bubalus bubalis) give a poor yield of milk but it has a high fat and protein percentage compared to taurine cattle. The identification of QTLs (Quantitative Trait Loci) on BTA14 and BTA6 and its subsequent fine mapping has led to identification of two non conservative mutations affecting milk production and composition. Our objective was to estimate the frequency of K232A (DGAT1 – diacylglycerol – acyltransferase 1) and Y581S (ABCG2 – ATP binding cassette sub family G member 2) polymorphisms in diverse cattle and buffalo breeds of India having large variation in terms of milk production.ResultsWe screened the reported missense mutations in six cattle and five buffalo breeds. The DGAT1K and ABCG2Y alleles were found to be fixed in Indian cattle and buffalo breeds studied.ConclusionThis study provides an indirect evidence that all the Indian cattle and buffalo breeds have fixed alleles with respect to DGAT1 and ABCG2 genes reported to be responsible for higher milk fat yield, higher fat and protein percent.

Highlights

Indian cattle (Bos indicus) and riverine buffalo (Bubalus bubalis) give a poor yield of milk but it has a high fat and protein percentage compared to taurine cattle
Grisart et al [6,7], Winter et al [8] and Weller et al [9] identified a polymorphism in exon 8 of the diacylglycerol – acyltransferase 1 (DGAT1) gene in Bos taurus, AA → GC exchange resulting in a non conservative substitution of amino acid 232 Lysine (K) → Alanine (A)
This polymorphism has been associated with increased fat yield, fat and protein percent as well as decrease in milk and protein

Summary

Introduction

Indian cattle (Bos indicus) and riverine buffalo (Bubalus bubalis) give a poor yield of milk but it has a high fat and protein percentage compared to taurine cattle. Grisart et al [6,7], Winter et al [8] and Weller et al [9] identified a polymorphism in exon 8 of the DGAT1 gene in Bos taurus, AA → GC exchange resulting in a non conservative substitution of amino acid 232 Lysine (K) → Alanine (A). This polymorphism has been associated with increased fat yield, fat and protein percent as well as decrease in milk and protein

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Conclusion