Abstract
Different region analysis (DFR-typing) was used for characterization of 275 Y. pestis strains isolated mainly from the CIS natural plague foci. Genomovars (DFR-types) endemic for 27 natural foci were determined. In addition to 32 genomovars described previously 64 novel Y. pestis DFR-types were revealed. 56 genomovars were shown to circulate in CIS and Mongolia. All strains analyzed by DFR-typing, were clustered in accordance with the phylogenetic tree proposed on the basis of combined SNP- and IS 100 -typing data. The discriminatory ability of the method is high enough to distinguish between subspecies, populations and even strains circulating in certain natural plague focus.
Highlights
Different region analysis (DFR-typing) was used for characterization of 275 Y. pestis strains isolated mainly from the CIS natural plague foci
In addition to 32 genomovars described previously 64 novel Y. pestis DFR-types were revealed. 56 genomovars were shown to circulate in CIS and Mongolia
All strains analyzed by DFR-typing, were clustered in accordance with the phylogenetic tree proposed on the basis of combined SNP- and IS100-typing data
Summary
В работе использовали 275 штаммов Y. pestis. Так как DFR01, DFR02 и DFR03 входят в состав плазмиды pFra [8], то для генотипирования использовали только штаммы, содержащие эту плазмиду. Геномную ДНК выделяли в соответствии с методическими указаниями МУ 1.3.1794-03 «Организация работы при исследованиях методом ПЦР материала, инфицированного микроорганиз-. DFR-анализ штаммов Y. pestis проводили, как описано ранее [6]. Поло жительные и отрицательные результаты амплификации фиксируют как «1» и «0» соответственно, что свидетельствует о наличии или отсутствии определенного DFR. 1. NJ-дендрограмма и DFR-профили 96 геномоваров Y. pestis: gv – геномовар; bv – биовар; ANT – antiqua (заглавные буквы обозначают, что геномовар описан ранее); INT – intermedium; med – medievalis; mic – microtus; ori – orientalis; ssp – подвид
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