Abstract
Objective: Synthesize silver nanoparticles using a green synthesis approach and encapsulate silver nanoparticles with a naturally occurring polymer, particularly of an-hydroglucose type, like dextran sulfate sodium salt and to study its anticancer activity.
 Methods: Green synthesis approach is been employed in the synthesis of silver nanoparticles using Psidium guajava leaf extract. The nanoparticles were then encapsulated with dextran sulfate biopolymer and the nanoparticles were subjected to different characterization techniques. The structure of the synthesized nanoparticles was analyzed using X-ray diffraction analysis, the presence of different functional groups was analyzed by FTIR studies. Size and morphology of the prepared nanoparticles were investigated using FESEM analysis. Anticancer activity of the synthesized nanoparticles was tested against the MCF-cell line.
 Results: The XRD analysis shows the crystalline nature of the synthesized nanoparticles. The stretching and vibrating modes of different functional groups were confirmed by FTIR result. The SEM image confirmed the presence of spherical shaped nanoparticles and the TEM image confirmed the average size of the particles to be around 24 nm. The Ag-DS NPs showed 91% cell inhibition for the concentration of 100 μg/ml, indicating the cytotoxicity of the nanoparticles against MCF-7 cell line.
 Conclusion: Dextran sulfate stabilized silver nanoparticles show potent anticancer activity against MCF-7 cell line.
Highlights
Nanoparticles are of great scientific interest among the research community because they form an effective bridge between bulk materials and atomic or molecular structures
Ag-DS NPs were synthesized at room temperature using Psidium guajava leaf extract as a reducing agent
The XRD analysis shows high intense diffraction peaks indicating the crystalline nature of synthesized Ag-DS NPs with face-centered cubic structure
Summary
Nanoparticles are of great scientific interest among the research community because they form an effective bridge between bulk materials and atomic or molecular structures. The use of silver nanoparticles for different applications depends on various factors including surface chemistry, size, size distribution, shape, particles morphology, particles composition, coating/capping, agglomeration, dissolution rate, particles reactivity in solution, efficiency of ion release and, type of reducing agent [4]. Dextran sulfate is a biopolymer having a well-defined structure that differs from a synthetic polymer having more random structure [7]. Silver nanoparticles or silver ions coordinate with carboxyl group and have a physical-steric interaction. Dextran derivative such as carboxymethyl dextran or dextran sulfate contains one or more carboxyl or sulpho groups, allowing conditions for forming complex compounds of different compositions and coordination with silver ions [8]
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